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143B細胞,ATCC人骨肉瘤細胞(CRL-8303)

  • 產品型號:
  • 產品時間:2024-08-08
  • 簡要描述:慧穎生物專業供應143B細胞,ATCC人骨肉瘤細胞(CRL-8303)細胞系,成纖維細胞,提供的細胞狀態良好,飽滿,光澤度好。慧穎生物擁有完善的細胞庫管理體系,售后及時到位。另有各類純進口胎牛血清,培養基,雙抗、胰酶,DMSO,生長因子等相關培養產品,種類齊全,*。!
  • 產品簡介

產品名稱:143B細胞,人骨肉瘤細胞系

來源:ATCC

貨號:CRL-8303

器官來源:骨

生長狀態:貼壁生長

規格:株

運輸狀態:凍存或常溫

相關疾病:骨肉瘤

價格:致電咨詢

143B細胞,ATCC人骨肉瘤細胞(CRL-8303),詳細培養資料及價格,點擊

來源:慧穎細胞庫

:王

咨詢:

細胞來源背景介紹:

Designations:

143B

Depositors:

 Wistar Institute

Biosafety Level:

1

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

adherent

Organism:

Homo sapiens

Morphology:

mixed


Source:

Organ: bone 
Disease: osteosarcoma

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

 

 

 

 

Applications:

transfection host

 

 

DNA Profile (STR):

Amelogenin: X 
CSF1PO: 12 
D13S317: 12 
D16S539: 10,13 
D5S818: 13 
D7S820: 11,12 
THO1: 6 
TPOX: 11 
vWA: 18

 

 

Age:

13 year old

 

 

Gender:

female

 

 

Ethnicity:

Caucasian

 

 

Comments:

Thymidine kinase negative (TK-). 
This is a human osteosarcoma cell line.

 

 

Propagation:

ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10%
Temperature: 37.0°C

 

 

Subculturing:

Medium Renewal: 2 to 3 times per week 
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

 

 

Preservation:

90% FBS; 10% DMSO

 

 

Related Products:

derivative:ATCC CRL-8304

 

 

References:

32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 870925632519: Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 862775833047: Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 866275733152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651

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